شماره مطلب: 457

Dietary manganese requirement of juvenile large yellow croaker Larimichthys crocea (Richardson, 1846)

نویسنده: 
Hanle Zhang
انتشارات: 
elsevier
تعدات صفحات: 
6
A 9-week feeding trial was conducted to determine the dietary manganese (Mn) requirement of juvenile largeyellow croaker Larimichthys crocea (Richardson, 1846) (initial mean weight 7.71 ± 0.02 g). Manganese sulfatemonohydrate (MnSO4·H2O) was added to the semi-purified basal diet to prepare 6 experimental diets withgraded levels of Mn (2.33, 7.63, 13.16, 18.27, 24.05 and 41.79 mg/kg, respectively). Results showed that the specificgrowth rate (SGR), feed efficiency (FE), Mn concentrations in whole-body, liver and vertebra, and activitiesof hepatic Mn superoxide dismutase (Mn-SOD) and total SOD (T-SOD) were significantly improved by dietaryMn supplementation (P b 0.05). However, fish fed the basal diet with 2.33 mg/kg of Mn had the significantlyhighest Mn retention, proportion of hepatic Mn to whole-body Mn and activity of copper–zinc superoxide dismutase(Cu–Zn SOD) (P b 0.05). As biomarkers of oxidative stress, malondialdehyde (MDA) and protein carbonyl(PC) in serumwere significantly higher in fish fed the basal diet (P b 0.05). Using the broken-linemodels based onSGR and hepatic Mn-SOD activity, dietary Mn requirements of the juvenile large yellow croaker were estimatedto be 16.44 and 16.16 mg/kg, respectively.
حجم: 
373KB

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