شماره مطلب: 488

Molecular and morphological analyses reveal that the pathogen Benedenia seriolae (Monogenea: Capsalidae) is a complex species: Implications for yellowtail Seriola spp. aquaculture

نویسنده: 
F.A. Sepúlveda
انتشارات: 
elsevier
تعدات صفحات: 
7
Monogeneans such as Benedenia seriolae are important pathogens of Seriola spp. that are farmed around theworld. In northern Chile, cultures of Seriola lalandi are being developed, and B. seriolae has been recorded infarmed fish and in natural populations. In this study, specimens identified as B. seriolae collected from farmedS. quinqueradiata in Japan were compared with specimens of B. seriolae collected from natural populations ofS. lalandi from the northern Chilean coast, and morphometrical and molecular analyses were used to evaluatewhether these samples corresponded to the same genetic group. Additionally, our sequences were comparedwith published sequences of Benedenia spp. from Australia. Themorphometrical characteristics of the specimensfrom Chile and Japan were evaluated using principal component analysis (PCA) and discriminant analysis (DA).Formolecular analyses, regions within the 28S ribosomal DNA large subunit (28S rDNA) and mitochondrial genecytochrome c oxidase 1 (COI)were used. Specimens of S. lalandi fromChile did not exhibit evidentmorphological(except by a slight difference in penis shape) and morphometrical differences compared with S. quinqueradiatafrom Japan. For 28S rDNA, the genetic distance between B. seriolae from Chile and Japan was 1.5%. For COI, thegenetic distance between B. seriolae from Chile and Japan was 14% and 15.2% for B. seriolae from Chile andAustralia. Similarly, the genetic distance between B. seriolae from Japan and Australia was 14.4%. Phylogenetictrees showed three clades: one group from Chile, one from Japan and one from Australia. Thus, ourresults indicate B. seriolae is a complex species, with three morphologically similar species, each one restrictedto a distinct geographic area. Given the differences in biological aspects such as fecundity and developmenttimes between species, an effective management strategy to prevent these monogenean epizootics mustbegin with accurate species identification, which should be considered previous to chemical treatments.Consequently, more biological information must be obtained for B. seriolae from the Chilean coast to understandits developmental timeline and infestation dynamics to control and manage this parasite in farmedyellowtail.
حجم: 
693KB

نظرات

  • دیدگاه های ارسال شده توسط شما، پس از تایید در وب منتشر خواهد شد.
  • پیام هایی که حاوی تهمت یا افترا باشد منتشر نخواهد شد.
  • پیام هایی که به غیر از زبان فارسی یا غیر مرتبط باشد منتشر نخواهد شد.